Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3

Abstract Sulforaphane (SFN), an active compound in cruciferous vegetables, has been characterized by its antiproliferative capacity. We investigated the role and molecular mechanism through which SFN regulates proliferation self-renewal of lung cancer stem cells. CD133 + cells were isolated with MACs from A549 H460 In this study, we found that inhibited simultaneously. Meanwhile, mRNA protein expressions Shh, Smo, Gli1 PHC3 highly activated Compared siRNA-control group, Knock-down Shh cells, decreased expression also affected level addition, activities Furthermore, inhibitory effect on siRNA-Shh siRNA-PHC3 was weaker than Sonic Hedgehog signaling pathway might undergo a cross-talk be effective new drug could inhibit modulation pathways PHC3. This study provide novel way to improve therapeutic efficacy for